It is required to utilize efficient separation procedures in order to study the function of
the different cell types in blood and bone marrow. The requirement is of paramount
importance in tissue typing in man. As is well known, transplantation of tissues
between individuals of an outbred species is mostly followed by rejection of the grafted
material. The rejection is very often accompanied by the appearance of humoral
antibodies, the specificities of which reflect the histocompatibility difference between
graft donor and recipient.
Majority of the presently available first in vitro assay techniques for examining the
antibodies and the iso-antigens which invoke their appearancc involve a subjective
interpretation of results, including that of microscopic examination of a cell population,
antigen, which has been damaged or agglutinated by antiserum. To meet the demand of
the present situation, precision and reproducibility in such an assay system, therefore,
would be most valuable.
The fundamental requirement is to obtain a homogenous cell suspension in any of the
reported assay systems. For the past few years, several investigations have been
reported, describing the preparation of lymphocytes from peripheral blood; and yet their
alleged high purity of the lymphocyte preparations seemed to be contaminated with other
leucocytes.
This paper reports a procedure of viable lymphocyte preparation virtually high in the
yield and purity.
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